The method of biomolecules immobilization is one of the key concerns in the fabrication of biosensors. The problem to be solved is to find immobilization strategies of biomaterials allowing their stability and reusability.
The choice of the immobilization method represents an important parameter that affects biosensor performances, mainly in terms of sensitivity, selectivity and stability, by influencing biomolecule orientation, loading, structure and biological activity.
Our research is focused on different immobilization strategies of proteins and biomolecules in biosensors, with applications in optical and electronic devices.
We use different immobilization strategies: physical adsorption in polyelectrolyte multilayers, entrapment in hydrogel or by means of overcoating, covalent bonds as well as combination of them.